Custom Services
Molecular biology
standard molecular cloning; PCR, RT-PCR, Quantitative real-time PCR diagnostics; RNAi technology, production of knock-down mammalian cell lines
Biochemistry
production of recombinant proteins; protein and protein complex purification (immunoaffinity, FPLC (Fast Pressure Liquid Chromatography), in vivo cross-linking); Western blotting; immunohistochemistry; immunofluorescence; immunoprecipitation
Cell biology
production of transgenic mice by the pronuclear injection of cloned DNA sequences; mouse embryonic stem cell culture; gene-targeting in mouse embryonic stem cells; production of knock-out mice; standard mammalian cell culture, transfection and manipulation; production of transgenic mammalian cell lines; isolation and culture of adult mesenchymal stem cells and embryonic stem cells; molecular characterization of stem cell lines by FACS (Fluorescence Activated Cell Sorting)
Cytology
FISH (Fluorescent In Situ Hybridisation) analysis; immunofluorescence; indirect immunostaining
Cell toxicity tests
Colorimetric endpoint assays: MTT dye conversion test, LDH release assay
Cell cycle and apoptosis analysis by FACS
Double staining of cell nucleus for detection of live/dead cells by confocal microscopy
Kinetic analysis of cell toxicity by real-time cell electronic sensing (RTCA) based on impedance
Calculation of toxic concentrarion (TC) 0, 50 and 100 values
The RTCA-SP system (ACEA Biosciences, Inc., USA) utilizes an automatical and continuous electronic readout called impedance to non-invasively quantify adherent cell proliferation and viability in real-time. Cells are cultured on 96-well E-plate containing gold microelectronic sensor arrays. The interaction between cells and electrode generates impedance response that correlates linearly with cell index reflecting cell number, adherence, cell growth and viability.
Measurement of compound permeability across biological barriers
Testing the bidirectional permeability of compounds and drug candidates across culture models of the blood-brain, intestinal and nasal barriers.
Schematic figure of bidirectional permeability measurement of compounds/drug candidates across culture models.
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Cell culture models of biological barriers
Blood-brain barrier model:
co-culture of primary brain endothelial and glial cells from rat or mouse
triple co-culture model of primary brain endothelial cells, pericytes and glial cells from rat or mouse (syngeneic)
Intestinal model: human intestinal cells*
Intranasal model: human nasal epithelial cells*
Lung model: human lung epithelial cells*
*The three epithelial models are available as monocultures or co-cultures endothelial cells
Co-culture and triple culture model of blood-brain barrier.
Research and Development
- Bioprocess design and engineering of biotherapeutics
- Drug targeting across blood brain barrier
- Blood-brain barrier model development
Consultancy
- Biotechnology and bioengeneering
- Blood-brain barrier permeability and drug targeting
- Toxicity testing on mammalian and human cells using 5 different methods